Caffeine-free coffee plants

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See the article in its original context from June 2003.

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Baltimore (AP) For those who love the full flavor of real coffee but can't handle the kick, the genetics revolution may have a solution.

Japanese researchers say they have genetically engineered coffee plants that have 70 percent less caffeine than usual in their leaves. The crucial question for brewing coffee whether beans from those plants will have less caffeine won't be known for three to four years when the plants mature, said study author Shinjiro Ogita.

However, the results indicate it should be possible, according to the researcher's report in Thursday's issue of the journal Nature.

The researchers at the Nara Institute of Science and Technology in Japan used RNA interference an increasingly popular genetic tool to manipulate the plant, interfering with the gene responsible for an enzyme used to make caffeine.

Experts contend a caffeinefree bean would be an improvement over current decaffeination processes, which use water or organic solvents to remove the stimulant from the beans before they are roasted, taking out some flavor and aroma as well.

Alan Crozier, a University of Glasgow researcher who has worked on genetically modifying coffee, said the Japanese group is the first to engineer the plants to produce less caffeine.

However, concerns about genetically modified foods and a lack of interest by the coffee industry could slow development, Crozier said. "I suspect it will come in first at the boutique end of the market and grow from there," Crozier said. "If it were to take over, clearly it's a much cheaper way to produce decaffeinated coffee."

Pablo Dubois of the London-based International Coffee Organization, which includes coffee-producing and consuming nations, said genetically modified foods "are regarded with wide suspicion in Europe" and current decaffeination processes are well established.

John Stiles, a scientist working to develop a caffeinefree coffee plant for Waialua, Hawaii-based Integrated Coffee Technologies Inc., said the Japanese researchers have not yet reached the commercial decaffeination level of 97 percent.

Stiles said the Hawaiian company hopes to have plants ready for field use in a year. While the Japanese researchers used the robusta variety of coffee plant, Stiles said the Hawaiian work uses the more commercial arabica variety.

Ogita said the Japanese researchers are also working on arabica plants and should be able to eventually remove all caffeine.

Coffee plants make caffeine in a three-step process. The targeted gene in the modified plant normally prompts the plant to produce an enzyme that carries out the second step, said Hiroshi Sano, one of the paper's authors.

RNA interference eliminates the chemical messenger the targeted gene sends to the cell's protein-making machinery.