Department of Environment enforcement officers charged four men on Wednesday afternoon in connection with the capture of a 400-pound turtle.
They had arrested one man on the morning of 1 October following the discovery of the dead turtle in an ice chest on board a local fishing boat.
They made three further arrests at 3.30pm Wednesday.
All four men were charged with possession of a turtle without a license and taking a turtle during the prescribed closed season.
They are scheduled to appear in Summary Court on Tuesday.
The turtle, which was four feet in length, was a fully grown female green sea turtle and was described by DoE officers as the largest they had seen.
Poaching turtles is illegal in the Cayman Islands at all times unless a person owns a DoE-issued turtling licence. Turtles are also protected during nesting season, which runs from April through November.